Coding

Part:BBa_K4743022:Design

Designed by: Jen Hsien, Liu   Group: iGEM23_PTSH-Taiwan   (2023-10-08)


bkt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1308
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 349


Design Notes

This sequence is optimized for saccharomyces cerevisiae

Source

Species: Chlamydomonas reinhardtii

Accession number:XP_001698699

References

1. Jin, J., Wang, Y., Yao, M., Gu, X., Li, B., Liu, H., Ding, M., Xiao, W., & Yuan, Y. (2018). Astaxanthin overproduction in yeast by strain engineering and new gene target uncovering. Biotechnology for biofuels, 11, 230. https://doi.org/10.1186/s13068-018-1227-4

2.Zheng, K., Wang, C., Xiao, M., Chen, J., Li, J., & Hu, Z. (2014). Expression of bkt and bch genes from Haematococcus pluvialis in transgenic Chlamydomonas. Science China. Life sciences, 57(10), 1028–1033. https://doi.org/10.1007/s11427-014-4729-8

3.Huang, J., Zhong, Y., Sandmann, G., Liu, J., & Chen, F. (2012). Cloning and selection of carotenoid ketolase genes for the engineering of high-yield astaxanthin in plants. Planta, 236(2), 691–699. https://doi.org/10.1007/s00425-012-1654-6